This chapter explains the technology and chemistry behind the IMx^® batch analyzer for the quantitative determination of a broad range of analytes. The system consists of an automated analyzer and reagent cells that are unique for each test. The immunoassays have a range of chemistries: microparticle capture, enzyme immunoassay, ion capture immunoassay, homogeneous and heterogeneous formats, and competitive and immunometric assay designs. The features, designs and principles are explained. There are also sections on calibration, antibodies, separation, signal generation and detection, and data processing, including interfacing to laboratory information systems.

This chapter is carried over from the third edition of The Immunoassay Handbook, and was written by Kent Ford of Abbott Diagnostics. It was reviewed and edited for the fourth edition by Frank Quinn and David Wild.

IMx^®, fluorescence polarization, microparticle capture, enzyme immunoassay, ion capture immunoassay, homogeneous, competitive, immunometric, calibration, antibodies, separation, signal generation, signal detection.